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Aims: Knowledge of optimal storage conditions of drugs is crucial for properly interpreting analytical assessments. Materials & methods: The current study aimed to investigate the stability of some nonsteroidal anti-inflammatory drugs using a validated method by gas chromatography (GC)–MS. For this propose, long-term, short-term and solution stability were investigated. Results: The analytes remained stable in […]
Aims: The drive toward more sensitive LC–MS assays has resulted in long, complex methods. We assessed next-generation trypsins to identify a suitable candidate to integrate into protein LC–MS method development strategies, to simplify methods and increase throughput. Materials & methods: The performance of commercially available next-generation trypsins was assessed based on the digestion of protein standards in buffer and complex matrix by LC–high-resolution MS. Results: The performance of all next-generation trypsins assessed exceeded that of an overnight tryptic
Background: Sodium oligomannate was approved for marketing by the National Medical Products Administration of China in 2019 for improving cognitive functions in mild-to-moderate Alzheimer’s disease patients. Method: LC–MS/MS methods were established and validated for the quantitation of sodium oligomannate in human plasma, urine and feces to support clinical development studies. Samples were prepared using liquid–liquid
Xu B and Haley R. Development and validation of methods that enable high-quality droplet digital PCR and hematological profiling data from microvolume blood samples. Bioanalysis 14(18), 1197–1211 (2022). The authors and editors of Bioanalysis regret any negative consequences this publication might have caused to the scientific community.
The following article has been retracted from Bioanalysis at the request of the authors. Read More »
Background: An antibody specific to small-molecule inhibitor-bound TNF has enabled the development of target occupancy biomarker assays to support the development of novel treatments for autoimmune disorders. Materials & methods: ELISAs were developed for inhibitor-bound and total TNF to determine the percentage of TNF occupancy in samples from stimulated blood. Inhibitor-saturated samples allowed measurement of
Aim: To develop a nondestructive method for the estimation of hematocrit (HCT) in dried blood spots (DBSs). Materials & methods: Standards and controls were created (HCT range: 0.20–0.50 l/l) and DBS scanned using a flatbed scanner. Gray values and pixel areas were analyzed with open-source software to estimate HCT and volume, respectively. HCT obtained in
Background: Indocyanine2,3-dioxygenase (IDO) is an enzyme that can catalyze the metabolism of tryptophan (Trp) into kynurenine (Kyn), thus inhibiting the tumor immune microenvironment. Method: Based on its inhibitor, NLG919 (NLG), the authors developed a new immunomodulatory polymer micelle and established and verified an ultrahigh performance liquid chromatography–mass spectrometry method for the simultaneous determination of NLG,
Jack Lodge, Commissioning Editor of Bioanalysis, and Neil Spooner, Editor-in-Chief, speak to Fumin Li. Fumin Li obtained his PhD in analytical chemistry from Iowa State University (ISU) in 2004. From 2004 to 2006, Li was a postdoctoral fellow at Pacific Northwest National Laboratory (PNNL), building ion mobility-mass spectrometry (i.e., IMS-TOFMS or FAIMS-TOFMS) and applying it
Introduction The 16th edition of the Workshop on Recent Issues in Bioanalysis (16th WRIB) was held on 26–30 September. Over 1000 professionals representing pharma/biotech companies, CROs, and multiple regulatory agencies convened to actively discuss the most current topics of interest in bioanalysis. The 16th WRIB included 3 Main Workshops and 7 Specialized Workshops that together
In contrast to quantification of biotherapeutics, endogenous protein biomarker and target quantification using LC–MS based targeted proteomics can require a much more stringent and time-consuming tryptic signature peptide selection for each specific application. While some general criteria exist, there are no tools currently available in the public domain to predict the ionization efficiency for a