Saliva, as the first body fluid encountering with the exogenous materials, has good correlation with blood and plays an important role in bioanalysis. However, saliva has not been studied as much as the other biological fluids mainly due to restricted access to its large volumes. In recent years, there is a growing interest for saliva […]
With the advent of the high-throughput technologies and exciting times for biology, the discipline of analytical methodology is experiencing a surge in the growth and the scope. Over the years, multitude of analytical techniques have evolved from a work-intensive, low sensitivity and high volume of reagent and sample consumption endeavor to automated, better selectivity, lower
Quantitative bioanalytical data are crucial in pharmaceutical research and development, allowing project teams to make informed scientific decisions on the progression of candidate molecules to medicines. Many challenges are often encountered during the bioanalysis of drugs in biological matrices which require resolution in a timely manner. In this publication, guidance is provided to bioanalytical scientists
Aim: Differential pulse polarography was used for the concurrent analysis of the coadministered dantrolene (DAN) and indomethacin (IND) in plasma. Materials & methods: DAN and IND, Hanging mercury drop electrode and Britton–Robinson buffer at pH 5 were used. In plasma, cathodic reduction of DAN nitro group and its active metabolite at -0.2 V was done.
Aim: Transdermal analysis is proposed for vitamin D3 and its hydroxylated metabolite to overcome problems associated with blood analysis. Methods: Vitamin D3 was extracted directly from skin with solid patches and liquid phases. Deuterium-labeled vitamin D3 was added to the extraction solutions to compensate for variability and accurately determine the rate of transdermal transfer. Of
The pharmaceutical industry has been working on improving drug development since decades. Currently, lack of efficacy and safety concerns are the major causes of attrition during late drug development. Under the current situation, biomarkers including pharmacodynamics (PD) and safety play an important role. Hayashi et al. proved that the use of a biomarker resulted in
VOLUME 11, ISSUE 2 / JANUARY 2019 Commentary NOVEMBER 2018 Biomarker assay validation for clinical trials: a questionnaire survey to pharmaceutical companies in Japan Yoshiaki Ohtsu, Takehisa Matsumaru, Masataka Katashima, Masaaki Kakehi, Hiroyuki Kakuo, Takayoshi Suzuki, Masanari Mabuchi, Ryosuke Nakamura, Takahiro Nakamura, Noriko Katori, Seiji Tanaka & Yoshiro Saito Pages:55–60 Published Online:26 November 2018 Buy PDF/ $200 Preliminary Communication
JANUARY 2019 | VOL. 11, NO. 2 Read More »
Aim: To develop a simple HPLC-DAD method for simultaneous determination of febuxostat (FEB) and diclofenac (DIC) in biological samples to assess pharmacokinetic outcomes of their coadministration. Methodology & results: Sample preparation was performed by liquid–liquid extraction. Drugs analysis was done on C18 column using methanol-formic acid pH 2.1 (76:24, v/v) as mobile phase and time-programmed
Aim: This study aims to develop a method for monitoring fat loss by detection of breath acetone. Methods: A new method combining direct breath sampling system and proton transfer reaction MS (PTR-MS) was developed for on-line detection of breath acetone. The breath acetone of 272 volunteers was detected respectively when exercise or diet. Results: Exercises
Aim: Trazodone (TZD) is used for the treatment of depression in adults and, off-label, as a sleep medication in adult and pediatric populations. The off-label use is well documented, however further clinical studies are needed to confirm its efficacy and safety for the treatment of sleep disorders. In this scenario, we developed a bioanalytical method