Aim: Monoclonal antibody-based treatment of cancer has been established as one of the most successful therapeutic strategies. Materials & methods: In this work, we developed a workflow based on an automated protein-A capture and LC–MS/MS analysis to quantify bevacizumab on patient serum during treatment. This analytical approach was fully validated and compared with a commercially […]
Aim: A validated LC–MS/MS assay for the quantitation of coproporphyrin-I and -III (CP-I, CP-III) in human plasma has been developed to understand the utility of both as possible endogenous biomarkers for organic anion-transporting polypeptides (OATP)-mediated drug–drug interactions (DDIs). Materials and Methods: Human plasma extracts were analyzed for CP-I and CP-III using a Sciex API 6500+
Background: N1-methylnicotinamide (1-NMN) has been proposed as a potential clinical biomarker to assess drug–drug interactions involving organic cation transporters (OCT2) and multidrug and toxin extrusion protein transporters. Results: A hydrophilic interaction liquid chromatography–MS/MS assay, to quantify 1-NMN, in human plasma and urine is reported. Materials & methods: A hydrophilic interaction chromatography (HILIC)-tandem mass spectrometry (MS/MS)
Aim: Evaluation of HPLC–high-resolution mass spectrometry (HPLC–HRMS) full scan with polarity switching for increasing throughput of human in vitro cocktail drug–drug interaction assay. Materials & methods: Microsomal incubates were analyzed using a high resolution and high mass accuracy Q-Exactive mass spectrometer to collect integrated qualitative and quantitative (qual/quant) data. Results: Within assay, positive-to-negative polarity switching
Aim: Selected bile acids (BAs) in plasma have been proposed as endogenous probes for assessing drug–drug interactions involving hepatic drug transporters such as the organic anion-transporting polypeptides (OATP1B1 and OATP1B3). Materials & methods: Plasma extracts were analyzed for selected BAs using a triple TOF API6600 high-resolution mass spectrometer. Results: Glycodeoxycholic acid 3-sulfate, glycochenodeoxycholic acid 3-sulfate,
Drug use during pregnancy constitutes a major preventable worldwide public health issue. Birth defects, growth retardation and neurodevelopmental disorders are associated with tobacco, alcohol or drugs of abuse exposure during pregnancy. Besides these adverse health effects, drug use during pregnancy also raises legal and social concerns. Identification and quantification of drug markers in maternal and
Ligand-binding assay (LBA) performance depends on quality reagents. Strategic reagent screening and characterization is critical to LBA development, optimization and validation. Application of advanced technologies expedites the reagent screening and assay development process. By evaluating surface plasmon resonance technology that offers high-throughput kinetic information, this article aims to provide perspectives on applying the surface plasmon
Aim: To implement pharmacokinetic drug monitoring and individualize the posology of new antiepileptic drugs, the first HPLC-diode array detection method was developed and validated to simultaneously quantify lacosamide, levetiracetam and zonisamide in human plasma. Materials & methods: Preceded by a reproducible liquid–liquid extraction, chromatographic separation was achieved by using a C18 column of 5 cm length and a mobile phase
Aim: There is a strong evidence that doxycycline can benefit abdominal aortic aneurysms patients because of its ability to inhibit matrix metalloproteinase enzymes. There is a need for a specific quantification method for doxycycline in these patients. We report herein the development and validation of a selective, specific, simple and rapid UHPLC–MS/MS method for doxycycline.
Urine is a biological matrix that contains hundreds of metabolic end products which constitute the urinary metabolome. The development and advances on LC–MS/MS have revolutionized the analytical study of biomolecules by enabling their accurate identification and quantification in an unprecedented manner. Nowadays, LC–MS/MS is helping to unveil the complexity of urine metabolome, and the results