December 2023
Dagmara Jacewicz,Aleksandra Dabrowska,Dariusz Wyrzykowski,Joanna Pranczk,Michal Wozniak,Jolanta Kubasik-Juraniec,Narcyz Knap,Kamila Siedlecka,Alexander J. Neuwelt andLech Chmurzynski
1Department of General and Inorganic Chemistry, University of Gdańsk, Sobieskiego 18/19, 80-952 Gdańsk, Poland
2Department of Medical Chemistry, Medical University of Gdańsk, Dębinki 1, 80-211, Gdańsk, Poland
3Department of Electron Microscopy, Medical University of Gdańsk, Dębinki 1, 80-211, Gdańsk, Poland
4Department of Histology & Immunology, Medical University of Gdańsk, Dębinki 1, 80-211, Gdańsk, Poland
5Department of Neurology, Oregon Health & Sciences University, Portland OR 97239, USA
*Author to whom correspondence should be addressed.
Abstract
The direct and accurate estimation of nitric dioxide levels is an extremely laborious and technically demanding procedure in the molecular diagnostics of inflammatory processes. The aim of this work is to demonstrate that a stop-flow technique utilizing a specific spectroscopic biosensor can be used for detection of nanomolar quantities of NO2 in biological milieu. The use of novel compound cis-[Cr(C2O4)(AaraNH2)(OH2)2]+ increases NO2 estimation accuracy by slowing down the rate of NO2 uptake. In this study, an animal model of pancreatitis, where nitrosative stress is induced by either 3g/kg bw or 1.5 g/kg bw dose of l-arginine, was used. Biochemical parameters and morphological characteristics of acute pancreatitis were monitored, specifically assessing pancreatic acinar cell death mode, NO2 generation and cellular glutathione level. The severity of the process correlated positively with NO2 levels in pancreatic acinar cell cytosol samples, and negatively with cellular glutathione levels.
Keywords: acute pancreatitis; nitrogen dioxide; l-arginine; Cr(III); rats; levels of inflammatory mediators; evaluation of apoptotic or necrotic effects