Aim: Volumetric absorptive microsampling offers a hematocrit insensitive way for microsampling. The aim of this study was to develop a method for routine healthcare for 14 antidepressants, antipsychotics and their active metabolites on a single-quadrupole HPLC–MS. A clinical validation study to determine conversion factors from capillary blood to plasma concentration was conducted afterward. Results: The method was validated according to current guidelines except for one substance. Five substances were measured in 49 patient samples and conversion factors could be derived with Passing–Bablok and Bland–Altman analysis. Conclusion: A reliable extraction and analysis method for commonly used antidepressants and antipsychotics was developed and validated. The method with the obtained conversion factors can now be used in routine healthcare.
Graphical abstract
The tip of the volumetric absorptive microsampling device is removed and put into a small reaction vessel. A prewetting step with 50 μl water for 15 min is followed by adding 250 μl of methanol with internal standard. The sample is then shaken for 10 min, sonicated for 15 min and shaken again for additional 10 min. After centrifugation, 130 μl of the supernatant are injected into the HPLC–MS.
Keywords:
- antidepressants
- antipsychotics
- clinical validation
- dried blood spots
- LC–MS
- Mitra
- therapeutic drug monitoring
- validation
- VAMS
- volumetric absorptive microsampling