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Abstract

Aim: A novel automated serial dried blood spot (DBS) sampler, ‘Fluispotter’, was tested for its sampling performance. Materials & methods: An LC–MS/MS method was developed for the analysis of cortisol in DBS samples serially spotted by Fluispotter. The cortisol concentrations in 148 paired DBS and plasma samples were compared across a hematocrit (HCT) range of 22–55%. Results: The interassay accuracy and precision were <10%. Overall assay bias was negligible across the HCTs tested when analyzing the whole-spot DBS samples. The accuracy and precision of the blood volume in 10 μl DBS samples spotted by Fluispotters and micropipettes were within 3%. Deming regression and Bland-Altman analysis showed a good agreement of DBS-predicted and measured plasma cortisol. Conclusion: The Fluispotter performed serial sampling with high accuracy and precision of the sample blood volume.

Graphical abstract

Keywords:

  • cortisol
  • dried blood spot
  • fluispotter
  • hematocrit
  • LC–MS/MS
  • microsampling
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