Aim: Novel compounds for obesity treatment are currently being studied employing lipidized analogs of anorexigenic neuropeptides. Various analogs of prolactin-releasing peptide have demonstrated their ability to decrease food intake. Adequate analytical tools are required to support corresponding research. Methodology & results: An analytical method was developed that includes simple dilution of plasma samples prior to liquid chromatography-mass spectrometry and employs a monolithic column for the determination of lipidized analogs of prolactin-releasing peptide in complex biological samples. A multiple reaction monitoring approach was applied that included matrix calibration and an internal standard and produced a linear calibration range 20–200 ng ml-1 in rat and macaque plasma samples. Conclusion: A straightforward, simple and reliable analytical method was developed satisfying major validation criteria.
Keywords:
- LC–MS
- lipopeptides
- monolithic column
- prolactin-releasing peptide
- stability