Aim: A pH-induced homogeneous liquid–liquid microextraction (HLLME) using a new switchable deep eutectic solvent has been used for the extraction of three antiepileptic drugs from breast milk samples. Methodology: This method is based on phase separation by changing pH. An ammonia solution and a phosphocholine chloride: hexanoic acid: p-aminophenol deep eutectic solvents were used as the phase separation agent and extraction solvent, respectively. Results: Significant factors were studied and the detection limits and enrichment factors were in the ranges of 0.009–0.19 ng ml-1 and 182–212 for the analytes, respectively. Also, linear ranges were wide (0.63–500 ng ml-1) and the method precision was acceptable. Conclusion: The introduced method was successfully applied for the determination of the analyte concentrations in breast milk samples.
Keywords:
- antiepileptic drugs
- breast milk
- deep eutectic solvent
- gas chromatography
- homogeneous liquid-liquid microextraction